Sepax
Zenix™ SEC Phases
Description
Zenix SEC 3µm phases are made of uniform, hydrophilic, and neutral nanometer thick proprietary
surface coating chemically bonded on high purity and mechanically stabilized silica. Zenix SEC 3µm
unique packing combined with large pore volume delivers highest and unrivaled separation efficiency
and resolution. The well-controlled surface chemistry results in excellent lot-to-lot reproducibility.
Our unique bonding chemistry, coupled with the maximized bonding density, allows Zenix SEC to provide
high stability and negligible non-specific interactions. The available pore sizes of Zenix packings are
100, 150 and 300Å. Typical applications for Zenix SEC columns include separation and analysis of
biological molecules and water soluble polymers in aqueous buffers.
Packing
- Particle size of 3 µm
- Selection of pore size: 100, 150 and 300 Å
- Highest separation efficiency and resolution
- High capacity
- High stability over low and high concentration salt
- Lot-to-lot reproducibility
- High protein recovery with intact biological activity
- Negligible non-specific interactions
- Ideal for separation and analysis of biological molecules: proteins, nucleic acids, oligonucleotides, peptides and virus
- Ideal for separation and analysis of natural polymers, e.g. polysaccharides, synthetic polymers, and nanomaterials, e.g. nanoparticles
| Phase | Zenix SEC-100 | Zenix SEC-150 | Zenix SEC-300 |
|---|
| Material | Neutral, hydrophilic film bonded silica |
|---|
| Particle size | 3 µm |
|---|
| Pore size | ~ 100 Å | ~ 150 Å | ~ 300 Å |
|---|
| Protein MW range (native) | 100 - 100,000 | 500 - 150,000 | 5,000 - 1,250,000 |
|---|
| pH stability | 2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.) |
|---|
| Backpressure (psi for a 7.8x300 mm) | ~ 1,500 | ~ 1,500 | ~ 1,500 |
|---|
| Maximum backpressure (psi) | ~ 4,500 | ~ 4,500 | ~ 3,500 |
|---|
| Salt concentration range | 20 mM - 2.0 M |
|---|
| Maximum temperature (°C) | ~ 80 |
|---|
| Mobile phase compatibility | Aqueous and organic |
|---|
SRT® SEC Phases
Description
Utilizing proprietary surface technologies, SRT SEC phases are made of the uniform, hydrophilic,
and neutral nanometer thick films chemically bonded on the high purity and enhanced mechanical
stability silica. This proprietary surface technology results in excellent column-to-column
reproducibility. The nature of the chemical bonding and the maximum bonding density of the
thin film benefit SRT SEC phases with high stability and negligible non-specific interactions.
Along with the widest pore size selection of 100, 150, 300, 500, 1000 and 2000Å, SRT SEC packings
have highest capacity that enables highest resolution.
Packing
- Uniform, nanometer thick molecule layer
- Chemically bonded to silica surface
- Hydrophilic
- Maximum bonding density
Antibodix™ Phase
Description
Antibodix columns are specially designed for high resolution, high efficiency and high
recovery separations of antibodies. The packing support is composed of a rigid, spherical,
highly cross-linked poly(styrene divinylbenzene) (PS/DVB) non-porous bead. The non-porous
resin has particle size of 1.7, 3, 5 and 10 µm. The PS/DVB resin surface is grafted with a
highly hydrophilic, neutral polymer thin layer with the thickness in the range of nanometer.
On the top of the hydrophilic layer, weak cation-exchange functional groups are attached via
a proprietary chemistry, resulting in high capacity ion-exchange layer.
Packing
| Product | Particle Size |
|---|
| Antibodix NP1.7 | 1.7 µm |
| Antibodix NP3 | 3 µm |
| Antibodix NP5 | 5 µm |
| Antibodix NP10 | 10 µm |
Proteomix® Ion exchange Phases
Description
Non-porous Proteomix Ion-exchange Phases - High Resolution and High Capacity
Proteomix ion-exchange phases have been innovatively developed for achieving high ion-exchange capacity,
a breakthrough technology for non-porous resins. Proteomix packing support is composed of a rigid, spherical,
highly cross-linked poly(styrene divinylbenzene) (PS/DVB) non-porous bead with the particle size selections
of 1, 1.7, 3, 5 and 10 µm. The PS/DVB resin surface is grafted with a highly hydrophilic, neutral polymer thin
layer with the thickness in the range of nanometer. The hydrophobic PS/DVB resin surface is totally covered by
such a hydrophilic coating that eliminates non-specific bindings with biological analytes, leading to high efficiency
and high recovery separations for biological molecules. On the top of the hydrophilic layer, ion-exchange functional
groups are attached via a proprietary chemistry, resulting in high capacity ion-exchange layer.
The uniqueness of non-porous Proteomix ion-exchange phases is that they combine increased
capacity and resolution with their intrinsic advantages of high efficiency and high separation speed.
Proteomics ion-exchange phases are especially suitable for high resolution, high efficiency and high
recovery separations of proteins, oligonucleotides, peptides, polysaccharides, cell lysates, nanoparticles
and nanotubes.
Porous Proteomix Ion-exchange Phases - Porous Proteomix packing support is composed of a rigid,
spherical, highly cross-linked poly(styrene divinylbenzene) (PS/DVB) particle with a pore size of 500 Å.
The surface is grafted with a highly hydrophilic, neutral polymer thin layer, and further chemically bonded
with a layer of ion-exchange functional groups. Four ion-exchange phases (SCX, WCX, SAX and WAX) are specially
designed for high capacity and high recovery separations of proteins, oligonucleotides, carbohydrates,
and peptides. The porous Proteomix resins have particle size of 5 and 10 µm for analytical separation,
and 30 and 50 µm for preparative separation.
Packing
The chemical structure of Proteomix ion-exchange phases is composed of a rigid PS/DVB core, a densely packed,
nanometer thick, hydrophilic coating, and a uniform ion-exchange layer, as shown in Figure 1.
As shown in Figure 2, Proteomix ion-exchange phases are composed of SCX, WCX, SAX, and WAX. The Proteomix
SCX phase is a strong cation exchanger with sulfonate functional groups chemically bonded to the top of the
hydrophilic coating for both porous and non-porous PS/DVB resins. The Proteomix WCX phase is a weak cation
exchanger with carboxylate functional groups chemically bonded to the top of the hydrophilic coating for both
porous and non-porous PS/DVB resins. The Proteomix SAX phase is a strong anion exchanger with quaternary ammonium
functional groups chemically bonded to the top of the hydrophilic coating for both porous and non-porous PS/DVB resins.
The Proteomix WAX phase is a weak anion exchanger with tertiary amine functional groups chemically bonded to the
top of the hydrophilic coating for both porous and non-porous PS/DVB resins.
Figure 1. Schematic illustration of the chemical
structure of Proteomix ion-exchange phases.
Figure 2. Chemical compositions of Proteomix SCX,
WAX, SAX, and WAX phases.
| Products | Pore Size | Particle Size | Dynamic Binding Capacity | pH range |
|---|
| Proteomix SCX-NP1.7,NP3,NP5,NP10 |
Non-porous |
1.7,3,5,10 µm |
~60,54,38,and 20 mg/mL |
2-12 |
| Proteomix SCX-POR10 |
500 Å |
10 µm |
~35 mg/mL |
2-12 |
| Proteomix WCX-NP1.7,NP3,NP5,NP10 |
Non-porous |
1.7,3,5,10 µm |
~25,19,15,and 10 mg/mL |
2-12 |
| Proteomix WCX-POR10 |
500 Å |
10 µm |
~22 mg/mL |
2-12 |
| Proteomix SAX-NP1.7,NP3,NP5,NP10 |
Non-porous |
1.7,3,5,10 µm |
~43,35,28,and 17 mg/mL |
2-12 |
| Proteomix SAX-POR10 |
500 Å |
10 µm |
~25 mg/mL |
2-12 |
| Proteomix WAX-NP1.7,NP3,NP5,NP10 |
Non-porous |
1.7,3,5,10 µm |
~35,26,18,and 12 mg/mL |
2-12 |
| Proteomix WAX-POR10 |
500 Å |
10 µm |
~20 mg/mL |
2-12 |
Glycomix™ SAX Phase
Description
Glycomix™ strong anion-exchange phase is made of narrow-dispersed and hydrophilic polymer particles.
It is a strong anion exchanger with quaternary ammonium functional groups that are chemically bonded
to the hydrophilic surface of the polymer particles.
Packing
Chemical composition of Glycomix™ SAX
| Characteristics | Glycomix™ SAX |
|---|
| Functional Group | Quaternary ammonium |
| Surface characteristics | Hydrophilic |
| pH range | 2 - 12 |
| Typical backpressure for 4.6x250 mm | 10 bar for 0.22 mL/min
38 bar for 1 mL/min |
| Maximum backpressure | ~ 1,500 (psi) |
| Salt concentration range | 20 mM - 2.0 M |
| Maximum temperature | ~ 80 (°;C) |
| Mobile phase compatibility | Aqueous and organic |
| Applications | Heparin, highly charged polysaccharides |
Carbomix® Phases
Description
Carbomix phases are composed of spherical, low cross-linking polystyrene/ divinylbenzene (PS/DVB)
particles with their surfaces modified with sulfonic acid (-SO3H), followed by chelating of calcium ions
(Ca+2) or other metal ions. The PS/DVB support has cross-linking degree of 5%, 8% and 10%. The standard
Carbomix packings for analytical applications are 5 µm uniform particles. Carbomix resins of 10 µm and
above particle size are manufactured for preparative separation applications. Carbomix resins have been
specifically designed for high resolution separation of carbohydrates, organic acids, peptides, and nucleic acids.
Packing
Carbomix Ca-NP resins have been specifically designed for high resolution separation of
carbohydrates, organic acids, peptides, and nucleic acids. These novel packing materials
are based on low crosslinked polystyrene / divinylbenzene (PS/DVB) particles with the
surface modified with sulfonic acid (-SO3H), followed by chelating of calcium ions (Ca+2).
Their narrow particle size distribution offers high efficiency and high resolution
separations. The low cross-linking generates swelling for the resin in the mobile phase,
resulting in reasonable surface area and capacity. Figure 1 is a typical test chromatogram
for the separation of carbohydrates by a Carbomix Ca-NP5 column.
Carbomix Ca-NP phases
| Support: | spherical, PS/DVB particles |
|---|
| Cross-linking: | 5%, 8% and 10% |
|---|
| Particle size: | 5 and 10 µm |
|---|
| Phase structure: | hydrophilic, ion-exchange |
|---|
| Chemical composition: | PS/DVB-(SO3-)2Ca2+ |
|---|
Carbomix H-NP resins have been specifically designed for high resolution separation
of carbohydrates, organic acids, peptides, and nucleic acids. These novel packing materials
are based on low crosslinked polystyrene / divinylbenzene (PS/DVB) particles with the
surface modified with sulfonic acid (-SO3H). Their narrow particle size distribution
offers high efficiency and high resolution separations. The low cross-linking generates
swelling for the resin in the mobile phase, resulting in reasonable surface area and
capacity.
Carbomix H-NP Series
| Support: | spherical, PS/DVB particles |
|---|
| Cross-linking: | 5%, 8% and 10% |
|---|
| Particle size: | 5 and 10 µm |
|---|
| Phase structure: | hydrophilic, ion-exchange |
|---|
| Chemical composition: | PS/DVB-SO3H |
|---|
Technical Specifications
| Phase | Carbomix Ca-NP | Carbomix H-NP |
|---|
| Support | Non-porous PS/DVB | Non-porous PS/DVB |
|---|
| Particle size | 5, 10 µm | 5, 10 µm |
|---|
| Cross-linking | 5%, 8% and 10% | 5%, 8% and 10% |
|---|
| Stationary phase | -(SO3)2-Ca2+ | -SO3H |
|---|
| pH stability | 5 - 9 | 1 - 3 |
|---|
| Typical backpressure (psi) | ~ 700 (0.6 mL/min for a 7.8x300 mm column) | ~ 700 (0.6 mL/min for a 7.8x300 mm column) |
|---|
| Maximum backpressure (psi) | ~ 2,500 | ~ 2,500 |
|---|
| Typical mobile phase | H2O | 5 mM H2SO4, H2O |
|---|
| Typical flow rate(mL/min) | 0.4-0.8 for a 7.8x300mm column
0.1-0.35 for a 4.6x300mm column | 0.4-0.8 for a 7.8x300mm column
0.1-0.35 for a 4.6×300mm column |
|---|
| Maximum temperature (°C) | ~ 85 | ~ 65 |
|---|
