FP-715 Fluorescence polarization Photometer

The model FP-715 is a powerful tool to analyze the equilibrium biomolecular binding profiles monitoring the rotational movement of molecules directly in solution by using Fluorescence polarization technology. Its operation is easy and simple because it does not require any filtration, immobilization or washing step. It is widely used in immunoassay, drug monitoring in clinical pharmacy, determining antibody specificity and affinity, and molecular interaction, including protein-protein, protein-DNA, coenzyme-protein, and antigen-antibody binding.

- Receptor/ligand studies (e.g. hormone/receptor assays)
- Protein/peptide interactions
- DNA/protein interactions
- Tyrosine Kinase Assays
- Competitive Immunoassays

The model FP-715 is a fluorescence polarization photometer designed to obtain measurement of both the degree of polarization (P) and anisotropy of fluorescence ( γ ). The P and γ values are used to evaluate the sample's degree of depolarization. The extent of depolarization reflects the micro viscosity or rotational diffusion of the dye-bound molecule. Such measurements have been used for analysis of system such as antigen-antibody reaction and cell membrance fluidity. Degree of polarization (P) and anisotropy of fluorescence ( γ ) data are obtained by measuring the emission intensity while automatically varying the orientation of the excitation polarizer from vertical to horizontal. The FP-71 5 is equipped with an automated polarizers for the EX monochromator

Specifications :

Hardware specifications

Light source: Xenon lamp with shielded lamp house, 75-watt

Detector: Photomultiplier

Monochromators

Excitation side Grating: Holographic grating with 1200 grooves/mm
modified Rowland mount

Wavelength range: 400 to 700 nm (5 nm step)

Spectral bandwidth: 10 nm
Emission side

Filter: Bandpath filter (530 nm provided as standard)

Spectral bandwidth: 40 nm

Polarizer and Analyzer

Excitation side: Automated polarizer for horizontal and vertical
polarization

Emission side: Manual polarizer for horizontal and vertical
polarization

Sample compartment

Applicable cells: 10 mm rectangular cell, 7.2 mm outer diameter or 10 mm outer diameter cylindrical micro cuvettes (Cylindrical cuvettes require optional cylindrical cuvette holders)

Sample volume: Minimum required sample volume

0.5 ml (7.2 mm dia. micro cell)

1.0 ml (10 mm dia. micro cell)

cell holder: Single cell holder

Temperature control: Peltier thermoelectric effect (air-cooled)

Temperature range: 15 to 60 degree celsius