SFC Analytique, Semi prep et préparative
chromatographie supercritique analytique
The use of supercritical fluid chromatography is a green alternative to traditional HPLC, with the use of CO2 taking the place of organic solvent as the mobile phase. CO2 has benefits for cost, and health & safety, as well as faster, cleaner sample recovery during sample purification. Other advantages of supercritical fluids include low viscosity with liquid-like densities, for higher solubility and increased column loading. The low viscosity and high diffusion lead to faster separations and faster extractions.
SFC has long been the method of choice for chiral separations, but with a large (and rapidly growing) range of column phases available – SFC is now becoming a powerful tool for achiral separations, both analytically and for sample prep.
The physical characteristics exhibited by supercritical fluid include a diffusion coefficient of dissolved molecules that is a hundred times greater than it is in liquid and a viscosity that is at least one figure smaller. A supercritical fluid chromatography system, which employs such a medium as a mobile phase, can be expected to serve as a separation analysis method that can rapidly perform separation without any drop in separation efficiency, even at fast flow rates, due to a rapid mass transfer inside the column when compared with high-speed liquid chromatography that uses liquid as the mobile phase.
In addition, when carbon dioxide is used as the medium, gasification will occur simply by keeping the separated and fractionated sample at a constant temperature, making this one of the techniques capable of highly efficient refining with few post-processing hassles, such as elimination solvents after preparative isolation. This offers a host of advantages, including cost savings related to the expense of purchasing solvent and discarding organic solvents, and high throughput analysis and preparative isolation due to rapid separation over a short period of time. As a gradient elution technique, SFC is able to vary the three parameters of pressure, temperature, and modifier solvent volume. It can be effectively used when separating oligomers and constituents with differing characteristics. (The temperature gradient requires the use of a column oven that continuously varies temperature over time.)
JASCO SFC System Advantage
Optimized
JASCO has been developing packed column SFC and SFE systems for more than 30 years; this evolution has resulted in a user-friendly, robust and powerful SFC-4000 Series.
Fast Response Back Pressure Regulator
The BP-4340 high-speed dynamic back-pressure regulator ensures an accurate and constant back pressure (typically within a few psi), regardless of flow rate and with virtually no hysteresis. The extremely low dead volume prevents fractions from remixing and precipitation build-up in the flow line.
Wide Range of Detectors
Fluorescence, UV-visible, Photometric Diode Array (PDA) and Chiral optical detectors. Non-optical detectors include FID, ELSD and single-quad mass-spec.
Find out more about the detectors here
Flexible
All SF-4000 series SFC systems can be used for HPLC – simply switch off the cooling and BPR.
Integrated Modular System
A basic system for each scale can be adapted to suit any application. From modifier selection to column heater type to detector combinations.
Compatible
JASCO SFC systems are compatible with most Mass Spectrometers and offer integrated control with both Advion CMS and Sciex Analyst.
Flexible Configurations
The three individual platforms for analytical, semi-prep and preparative scales can be customized to suit all applications.
SFC Semi preparative
Analytical to Semi-preparative Flow Rates
Using columns from 4.6 to 10 mm ID, the SP-4000 Semi-Preparative SFC System can be used for separation and purification up to several 100 mg per day of sample material.
Typical Purification range
Analytical and semi-preparative flow rates from 1mL/min to 20mL/min for purification of samples up to 100s of mg
Optional Modifier Pumps
Optional two flow rate scale modifier pumps for fine tuning modifier composition.
Column Switching
Six or ten column switching for automatic selection for method development.
Solvent Switching
Six or ten modifier solvent selection for method development.
High-Capacity Column Oven
High capacity column oven for up to 6 x 10mm columns, with an option for use of 3 column ovens for even greater flexibility.
High Recovery Rate
Patented individual MCS MicroCyclone fraction collection system for sample collection without cross contamination.
Powerful Software Options
Two software options include ChromNav and SF-Nav for simple control with powerful fractionation algorithms
Wide Range of Detectors
Wide range of detectors including UV-Visible, PDA, Mass Spec, ELSD, FID and Circular Dichroism. And for applications requiring the utmost sensitivity, JASCO has designed the first high pressure flow cell designed for our fluorescence detectors making them compatible with SFC.
High-Throughput Fraction Collection System
Fraction collection with 8 fraction valve or open bed fraction collector.
Dynamic Back Pressure Regulator
Patented dynamic back pressure regulator for accurate control of pressure for accurate and reproducible separations.
Supercritical Fluid Extraction (SFE) Systems
Système SFE
Supercritical Fluid Extraction (SFE) typically uses supercritical carbon dioxide as the extraction solvent of choice as it has many advantages. Extraction using supercritical CO2 has the benefit of high matrix penetration and low viscosity at relatively low temperatures and in the absence of oxygen, this is important for compunds that may be heat labile or easily oxidized. SFE offers short extraction times and require little or no organic solvent compared with other extraction techniques.
Applications for SFE include the extraction of active ingredients, including various flavors and medicinal constituents from natural product e.g. docosahexaenoic acid (DHA), advanced unsaturated fatty acids and fatty esters such as eicosapentaenoic acid (EPA), fat-soluble vitamins and pharmaceuticals. Other applications include the elimination of unwanted constituents, such as decaffeination and desolvation in pharmaceutical tablets. It can also be applied to the pre-processing of samples prior to separation using HPLC or GC.
Supercritical Fluid Extraction System – Configuration
This system comprises a supercritical carbon dioxide pump, a modifier co-solvent pump, extraction vessel, and an automatic back pressure regulation valve. We offer a wide variety of extraction vessels in a range of different sizes and they can either be a column or cup type, depending on the physical state of the extraction sample. Extraction parameters include solvent volume, pressure, temperature and co-solvent ratio all of which can be adjusted to suit the application for optimal extraction yields.
Residual Pesticide Extraction Systems
SFE is an excellent technique for analyzing trace pesticides in soil and on food products. Residual pesticide extraction systems that use supercritical carbon dioxide are extremely versatile. JASCO has several SFC systems dedicated to residual pesticide extraction.
- Extraction vessel sizes : 10 mL or 50 mL
- Simple patented ‘Fasten Lock‘ attachment offers simple loading and removal for both sizes of vessel
- One to six vessels can be batch processed
- Processing method for multiple samples. Continuous processing (serial) is offered as standard. Parallel processing is supplied as an option
| System 1 | System 2 | System 3 | System 4 | System 5 | |
|---|---|---|---|---|---|
| System Features | Full-automatic High-throughput 2 flow lines with parallel processing | Full-automatic Single flow line | Full-automatic Single flow line | Manual 2 flow lines with parallel processing | Manual 2 flow lines with parallel processing |
| CO2 flow rate | Max. 20 mL/min | Max. 20 mL/min | Max. 10 mL/min | Max. 20 mL/min | Max. 10 mL/min |
| Operating pressure | Max. 30 MPa | Max. 30 MPa | Max. 30 MPa | Max. 30 MPa | Max. 30 MPa |
| Extraction temp. | Ambient + 10ºC to 80ºC | Ambient + 10ºC to 80ºC | Ambient + 10ºC to 80ºC | Ambient + 10ºC to 80ºC | Ambient + 10ºC to 80ºC |
| Extraction vessel | 50 mL | 50 mL | 10 mL | 50 mL | 10 mL |
| Vessel handling | Fasten Lock | Fasten Lock | Tubing and connectors | Fasten Lock | Tubing and connectors |
| Sample number | 6 | 6 | 6 | 2 | 2 |
| Collection method | Trap column | Trap column | Trap column | Trap column | Trap column |
Features
- Simple operation
- Quick extraction – about 30 to 60 minutes per sample, which is one-third to one-forth the processing time of conventional techniques
- Eliminates trace pesticides – the extract is collected in a trap column
- Minimal extract cleanup – also roughly separates impurities in trap column
- Blockages and contamination are minimized – the trap column and piping is washed each tim
- Low running costs – carbon dioxide is highly pure and inexpensive
- The system is safe and environmentally friendly because carbon dioxide is non-toxic and nonflammable
Flexibility
- Capacity of extraction vessels: 10 mL or 50 mL. The 10 mL vessel is ideal if operability is the focus. The 50 mL vessel is a better selection if the focus is on sample processing volume or analysis sensitivity
- The 50 mL vessel offers simple one-touch attachment and removal
- One to six vessels can be processed at a time. JASCO offers the single vessel type or the six-vessel type depending on the frequency of samples being measured
PR-4088 Prep SFC Features
SFC Preparative
Fast CO2 Delivery
Preparative scale CO2 delivery of up to 150mL/min, or up to 200mL when combined with a modifier co-solvent.
Maximum Sample Preparation
Using columns from 10 to 30 mm ID, the PR-4088 Prep System can be used for separation and purification from 100mg to 100g per day of sample material.
Sample separation for Chiral & Achiral
PR-4088 Prep System is excellent for both Chiral and Achiral sample preparation.
Powerful SFC Management Tool
Using SF-Nav software the PR-4088 Prep can be used with a wide variety of fraction collection methods, including time window, slope and/or threshold, to achieve high throughput stack injection.
Complete Audit trail to monitor campaigns
SF-NAV controls your campaign; monitor the individual chromatograms, complete sets of stacked injections, pressure and temperature profiles throughout the system.
High-Throughput Sample
Several fraction collection regimes for use with larger sample bottles for larger scale purification.
Accurate Chromatography with Large Volume Injections
PR-4088 uses the Patented DLI (Dual Line Injection) to ensure excellent chromatography when making large volume injections.
8 Fraction Sample Collection Unit
The PR-4088 fraction collection unit includes the patented dynamic back pressure regulator, 8 individual heated cyclone separators with a separate waste line. Fraction control allow sequential or stacked injection, with the option to collect the fractions between peaks to ensure there is no sample loss. Recoveries are typically in the region 95% or better with negligible cross contamination between fractions.
Widest Range of Detection Schemes
The PR-4088 SFC System can be used with a single detector or combination of detectors to control fractionation, including Mass Spec, UV-Vis, PDA and chiral (Circular Dichroism). And for applications requiring the utmost sensitivity, JASCO has designed the first high pressure flow cell designed for our fluorescence detectors making them compatible with SFC.
