Description
Zenix SEC 3µm phases are made of uniform, hydrophilic, and neutral nanometer thick proprietary surface coating chemically bonded on high purity and mechanically stabilized silica. Zenix SEC 3µm unique packing combined with large pore volume delivers highest and unrivaled separation efficiency and resolution. The well-controlled surface chemistry results in excellent lot-to-lot reproducibility. Our unique bonding chemistry, coupled with the maximized bonding density, allows Zenix SEC to provide high stability and negligible non-specific interactions. The available pore sizes of Zenix packings are 100, 150 and 300Å. Typical applications for Zenix SEC columns include separation and analysis of biological molecules and water soluble polymers in aqueous buffers.
Packing
- Particle size of 3 µm
- Selection of pore size: 100, 150 and 300 Å
- Highest separation efficiency and resolution
- High capacity
- High stability over low and high concentration salt
- Lot-to-lot reproducibility
- High protein recovery with intact biological activity
- Negligible non-specific interactions
- Ideal for separation and analysis of biological molecules: proteins, nucleic acids, oligonucleotides, peptides and virus
- Ideal for separation and analysis of natural polymers, e.g. polysaccharides, synthetic polymers, and nanomaterials, e.g. nanoparticles
| Phase | Zenix SEC-100 | Zenix SEC-150 | Zenix SEC-300 |
|---|---|---|---|
| Material | Neutral, hydrophilic film bonded silica | ||
| Particle size | 3 µm | ||
| Pore size | ~ 100 Å | ~ 150 Å | ~ 300 Å |
| Protein MW range (native) | 100 – 100,000 | 500 – 150,000 | 5,000 – 1,250,000 |
| pH stability | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | ||
| Backpressure (psi for a 7.8×300 mm) | ~ 1,500 | ~ 1,500 | ~ 1,500 |
| Maximum backpressure (psi) | ~ 4,500 | ~ 4,500 | ~ 3,500 |
| Salt concentration range | 20 mM – 2.0 M | ||
| Maximum temperature (°C) | ~ 80 | ||
| Mobile phase compatibility | Aqueous and organic | ||
